Best Paper of the Session & Col.Rangachari Contestant

DR. THIRUMALESH M. B.

Prof.ROHIT SHETTY, Dr. POORNACHANDRA B.

Abstract

Aim:To investigate role of ICAM &effect of Intravitreal (IV) small molecule which inhibits ICAM mediated effect vs Ranibizumab (RBZ) in preventing vascular leakage in Streptozocin (STZ) induced diabetic mouse model (DMM). Methods: Aqueous humor of DME patients had proinflammatory cytokines (IL6&IL8), VEGF &elevated levels of sICAM. In-vitro study on RPE&endothelial cell lines subjected to glycemic stress showed cell surface expression of ICAM hence sICAM1 used as lead target for mechanistic exploration in-vivo in STZ induced DMM. Results: RWJLFA1antagonist showed significant reduction in leakage patterns which was comparable RBZ treated showing sICAM role. ICAM1,IL6 &PEDF expression is reduced post RWJLFA1antagonist treatment. Anti-VEGF able to achieve reduction in mRNA expression of all genes. Conclusion: This translational study shows that RWJLFA1 antagonist is potential drug target &can be used as novel treatment option for DME.

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