Dr. SIDDHARTH NARENDRAN

Dr. LALITHA PRAJNA, Dr.PRAJNA N.V.

Abstract

Purpose : Clinical Evaluation of a CRISPR/Cas12a-based Nucleic Acid Detection Platform ( Rapid Identification of Mycoses using CRISPR, RID-MyC Assay) for the Diagnosis of fungal endophthalmitis (FE).

Methods: Isothermal amplification was performed using RPA followed by the CRISPR/Cas12a reaction. Intraocular specimens were collected from 25 control patients and 60 patients with suspected endophthalmitis. The results of the RID-MyC assay were compared to microscopy, culture, conventional PCR, real-time PCR and ITS sequencing.

Results: The positive concordance and negative concordance rate between PCR and RID-MyC was analyzed. RID-MyC correlated better with the clinical interpretation and sequencing results than PCR. The sensitivity of RID-MyC for the diagnosis of fungal endophthalmitis was significantly (p < 0.05) better than culture and microscopy. Conclusion: CRISPR-mediated detection of fungal nucleic acids is a rapid, highly sensitive and specific tool for the diagnosis of FE.

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