Dr.DEEPIKA C P

Dr. BRIJESH TAKKAR

Abstract

This is a laboratory experimental study wherein the RPE cell suspensions were injected via two different bores of cannulas of inner bore sizes of 29G and 41G. Percentage cell viability or cell death was evaluated by Trypan blue dye exclusion test and cell counting using a haemocytometer. We evaluated two different concentrations of cell suspensions namely, 10 million/mL and 2 million/mL. In case of 10 million/mL cell suspension, the pre injection cell viability of 98.88% is reduced to 95.96% post injection with a 41G cannula and to 95.38% post injection with a 29G needle. Similarly, for 2 million/mL cell suspension, the pre injection cell viability of 98.88% is reduced to 83.87% post injection with a 41G cannula and to 94.92% post injection with a 29G needle. This in vitro study results have confirmed that the viability of RPE cell suspension injection via 41G cannula is comparable to that of 29G cannula and maintained an average viability index of >90% post-injection.

Full Text